The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Protoplast Isolation and Reversion from Lyophyllum ulmarium

만가닥버섯의 원형질체 분리(分離) 및 환원(還元)

  • Yoo, Young-Bok (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • You, Chang-Hyun (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • Park, Yong-Hwan (Applied Mycology and Mushroom Division, Institute of Agricultural Sciences, R.D.A.) ;
  • Chang, Kwon-Yawl (Department of Agronomy, College of Agriculture, Gyeongsang National University)
  • 유영복 (농촌진흥청 농업기술연구소 균이과) ;
  • 유창현 (농촌진흥청 농업기술연구소 균이과) ;
  • 박용환 (농촌진흥청 농업기술연구소 균이과) ;
  • 장권열 (경상대학교 농과대학 농학과)
  • Published : 1987.03.30
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Abstract

This experiment was undertaken to investigate proper conditions for protoplast formation from Lyophyllum ulmarium. Combination of Novozym 234, ${\beta}-Glucuronidase$ and ${\beta}-D-Glucanase$ with 0.6 M Sucrose was the most effective for isolation of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs in shaking condition at 120 strokes $min-^1$. When the mycelium of L. ulmarium was cultured for 6 days on yeast glucose agar medium at $25^{\circ}C$, the formation of protoplasts was effective. The yeast glucose agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.

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