Tumour Specific and Immunosuppressive Components in Soluble Cell Extracts from Ovine Squamous Cell Carcinoma

면양(緬羊) Squamous Cell Carcinoma의 세포추출액(細胞抽出液)중에 함유된 종양특이(腫瘍特異) 및 면역기능저하물질(免疫機能低下物質)

The ovine squamous cell carcinoma (OSCC)-specific and immunosuppressive properties of OSCC extracts were investigated by using the techniques of lymphocyte blastogenicity, acid dissociation-ultrafiltration and gradient polyacrylamide gel electrophoresis. It was found that OSCC extracts contained two major and one minor protein peaks by Sephadex gel fractionation. Two major peaks bear substantial amount of immunoglobulins, antigen-antibody complex and OSCC-specific fractions, and the minor peak includes immunosuppressive materials. OSCC-specific components were detected at the molecular weights of 10,000 to 100,000 daltons in the major peaks and immunosuppressive materials at the fractions with the molecular weight of 10,000 to 100,000 and < 10,000 daltons in the minor peak. When the fractions were further separated by gradient polyacrylamide gel electrophoresis, the OSCC-specific antigens were found in the slice number 4 to 6 in fraction III, and immunosuppressive materials, in the slice numbers 9 to II in fraction V. The present results were considered to provide a basis for preparation and purification of OSCC-specific and immunosuppressive materials from the crude OSCC extracts.

3M KCl을 이용하여 면양 squamous cell carcinoma 및 정상면양 조직(組織)에서 획득(獲得)한 추출액(抽出液)의 성상을 sephadex column chromatography, lymphocyte blastogenicity assay, acid dissociation method 및 gradient polyacrylamide gel electrophoresis 등을 이용하여 연구하였다. Sephadex column chromatography에서 얻은 5개의 종양분획(腫瘍分劃)중 분획(分劃)III은 가장 종양특이(腫瘍特異)한 항원(抗原)을 보유하고, 분획(分劃)V lymphocyte reactivity를 저하(低下)시키는 성분을 함유하고 있었다. 각 분획(分劃)을 분자량(分子量) > 100,000, 10,000~100,000 및 < 10,000의 3군(群)으로 분리한 바 종양특이항원(腫瘍特異抗原) 및 면역저지물질(免疫沮止物質)은 10,000~100,000분획(分劃)에서 나타났다. 분획(分劃)I tumour specific antigen - antibody complex를 내포하고 있음이 밝혀졌다. Gradient gel electrophoresis를 이용하여 분획(分劃)을 더욱 세분했을 때 분획(分劃)III은 Slice No 4~6에서 종양특이물질(腫瘍特異物質)이 인정되었고, 분획(分劃)V Slice No 9~11에서 면역기능저하물질(免疫機能低下物質)이 있음이 밝혀졌다.