농업과학연구 (Korean Journal of Agricultural Science)
- 제13권2호
- /
- Pages.185-192
- /
- 1986
- /
- 2466-2402(pISSN)
- /
- 2466-2410(eISSN)
영지(靈芝)버섯 균사(菌絲)의 원형질체(原形質體) 나출(裸出) 및 재생(再生)
Some Factors Affecting the Protoplast Formation and Regeneration from the Mycelium of Ganoderma lucidum(Fr.) Karsten
- Shin, Gwan Chull (Dept. of Agricultural Biology, Coll. of Agriculture, Chungnam Nat'l Univ.) ;
- Yeo, Un Hyung (Dept. of Agricultural Biology, Coll. of Agriculture, Chungnam Nat'l Univ.) ;
- Yoo, Young Bok (Institute of Agricultural Sciences) ;
- Park, Yong Hwan (Institute of Agricultural Sciences)
- 발행 : 1986.12.31
초록
영지(靈芝)버섯의 유전(遺傳) 및 신품종(新品種) 육성(育成)을 위한 기초연구(基礎硏究)로서 원형질체(原形質體) 나출(裸出)에 미치는 몇가지 요인(要因) 구명(究明)하고 원형질체(原形質體)의 재생(再生)에 관한 실험(實驗)을 실시(實施)한 결과(結果)는 다음과 같다. 1. 영지(靈芝)버섯균(菌)의 원형질체(原形質體) 나출(裸出)에 적합(適合)한 배지(培地)는 균사생장(菌絲生長)이 빠르고 기생균사(氣生菌絲)의 발달(發達)이 양호(良好)한 SCM이었다. 2. 영지(靈芝)버섯균사(菌絲)의 세포벽(細胞壁) 분해효소(分解酵素)는 Novozym 234였고 최적농도(最適濃渡)는
Some factors affecting the protoplast release from mycelia of Ganoderma lucidum and regeneration of the protoplast were investigated and the results obtained are summarized as follows; Novozym 234 as a lytic enzyme was the most effective for the protoplast release from mycelia of Ganoderma lucidu m and its optimal concentration was 10mg per ml of osmotic stabilizer. The highest number of protoplasts were released after 3 hours incubation in the reciprocal shaking bath at 120 oscillations a minute. Among six osmotic stabilizers tested, 0.6M sucrose showed the best result. SCM medium showed good mycelial growth and high yields of protoplasts. The protoplasts released from the mycelium of G. lucidum were regenerated at 0.20 to 0.27 percent on MCM, MMM and SCM. Of the cultures obtained from protoplasts regenerated, 13 to 29 percent were monokaryon.
키워드