The Journal of the Korean Society for Microbiology (대한미생물학회지)
- Volume 21 Issue 1
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- Pages.163-170
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- 1986
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- 0253-3162(pISSN)
Proliferation and Characteristics of Murine T Lymphocytes in A Mitogen-Induced Conditioned Media(Interleukin-2)
마우스 T세포 증식인자(interleukin-2)에 의한 마우스 T림프구의 장기배양 및 그 특성
- Nam, Sang-Yun (Immunology Research Laboratory, Kyung Hee University Hospital) ;
- Ha, Youn-Mun (Department of Microbiology, Kyung Hee University, School of Medicine) ;
- Choi, Yong-Mook (Immunology Research Laboratory, Kyung Hee University Hospital)
- Published : 1986.03.31
Abstract
Balb/c mouse spleen cells in vitro sensitized against ICR spleen cells were cultured in conditioned media(CM). The CM was produced by ICR spleen cells stimulated with Concanavalin-A(Con-A), and sensitized lymphoid cells were grown in CM. ICR mouse spleen cells were appeared to be a good generator of IL-2. Optimal growth was seen in growth medium containing 20% fetal calf serum. and 25% CM. When cultures were initiated at 1, 5,
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