Studies on the leaf Color and Absorbance in Visible Range of Rice Plant

벼 엽색과 가시부의 흡광도와의 관계

The rice leaf color, which was one of main factors to determine application time of fertilizers, was analyzed in visible region, while the distinction of photosynthetic pigments controlling the rice leaf color was separated by T.L.C. The light absorption peaks of ethyl ether extracts of rice leaf blades were occurred in 413, 432, 453 and 662nm. Especially, the maximal light absorption was 432nm in blue and 662nm in red region. The both light absorption ratios (blue to red region) was not greatly different among rice ecotypes, whereas decreasing tendency in increased fertilization was observed. There was a significant negative correlation between the both light absorption ratios and the values of color chart, and the content of chlorophyll in rice leaf blades. The composition ratio of chorophyll was larger than that of carotenoid at heading stage, and the carotenoid was larger than the chlorophyll at 30 days after heading. The composition ratio of chlorophyll among photosynthetic pigments according to rice ecotypes, Japonica x Indica cultivars were larger than Japonica cultivars at heading stage, and Japonica cultivars were larger than Japonica x Indica cultivars at 30 days after heading, while carotene was vice versa.

벼의 엽색은 시비시기를 결정하는데 주요한 인자로써 가시부 영역에서 해석하고, 이들 엽색을 지배하는 광합성색소는 T.L.C.로 분리하여 검토하였다. 1. 벼의 엽색은 413, 432, 453nm(청색부)와 662nm(적색부)에서 흡광되였고, 그 중에서 최대흡광은 432, 662 nm 이었다. 2. 양흡광도비(청색부/적색부)는 벼 생태형간에는 근 차이는 없었으나. 비료간에는 비료양이 증가할수록 적었다. 3. 양흡광도비와 엽색도 및 엽록소함량과는 부의 유의상관이 인정되었다. 4. 광합성색소 조성비율은 출수기에서는 녹색계통색소, 출수후 30일에는 황색계통색소의 조성비율이 각각 컸다. 5. 벼 생태형별 광합성색소율 Chl. a의 조성비율은 출수기에는 다수계품종이 일반계품종보다 컸고, 출수후 30일에는 일반계품종이 다수계품종보다 컸으나, carotene는 그 반대이었다.