한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제14권3호
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- Pages.213-218
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- 1986
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
YRp7 vector를 이용한 Bacillus amyloliquefaciens amylase gene의 cloning I I. Saccharomyces cerevisiae에서 발현
Cloning of Bacillus amyloliquefaciens amylase gene using YRp7 as a vector II. Expression of cloned amylase gene in Saccharomyces cerevisiae
초록
B. amyloliquefaciens의
Hybrid plasmid pEA24, shuttle vector YRp7 carrying amylase gene of Bacillus amyloliquefaciens, was transformed to yeast Saccharomyces cerevisiae, and the expression of B. amyloliquefaciens amylase gene in yeast was investigated. The frequency of transformation to S. cerevisiae DBY747 with YRp7 was increased by treatment of 40% polyethylene glycol (MW 4, 000), PH 7.0, at 3