Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 14 Issue 2
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- Pages.139-143
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- 1986
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Purification and enzyme characteristics of laccase from Ganoderma lucidum
Ganoderma lucidum 균주에 의한 Laccase의 정제 및 효소적 특성
Abstract
The production media and enzymatic characteristics of laccase from Ganoderma lucidum was investigated. Potato dextrose yeast extract media was proved to be the best for laccase production. The enzyme has optimum pH of 6.45km value of 6.71 mM and appeared to be stable at wide pH range. The enzyme was inactivated partially by methanol and ethanol and totally by sodium azide but not at all by acetone. Also the enzyme purification was performed and the data is given.