Korean Journal of Microbiology (미생물학회지)
- Volume 24 Issue 4
- /
- Pages.406-410
- /
- 1986
- /
- 0440-2413(pISSN)
- /
- 2383-9902(eISSN)
A new restriction endonuclease from xanthomonas citri
새로운 type II 제한효소 xci I의 분리
- Whang, Y. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Chae, K. S. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Jang, W. H. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
- Kim, K. T. (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology) ;
-
Yoo, O. J.
(Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology)
- 황영 (한국과학기술원 생물공학과) ;
- 채건상 (한국과학기술원 생물공학과) ;
- 장원희 (한국과학기술원 생물공학과) ;
- 김기태 (한국과학기술원 생물공학과) ;
-
유욱준
(한국과학기술원 생물공학과)
- Published : 1986.12.01
Abstract
The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity.