.betha.-1, 3-glucanase 생성균의 분리 및 효소 생성 조건

Isolation of .betha.-1, 3-glucanase producing strain and cultural conditions of its enzyme production

  • 정기택 (경북대학교 식품가공학과) ;
  • 방광웅 (경북대학교 식품가공학과) ;
  • 송형익 (경북대학교 식품가공학과) ;
  • 김재근 (경북대학교 식품가공학과) ;
  • 유대식 (계명대학교 생물학과)
  • 발행 : 1986.09.01

초록

The bacteria, which were capable of producing ${\beta}-1$, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. the enzyme production was reached maximum state shen the broth cultured for 72hr at $30^{\circ}C$. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% $MnSO_4$ at pH 7.5.

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