미생물학회지 (Korean Journal of Microbiology)
- 제24권2호
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- Pages.106-112
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- 1986
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Pseudomonas putida로 부터 salicylate 분해 plasmid의 분리
Isolation of Salicylate-Degrading Plasmid from Pseudomonas putida
초록
자연계에서 분리한 Pseudomonas에서 크기가 매우 큰 plasmid (약 180 megadaltons:를 분리하였다. 이 plasmid는 기존의 plasmid 검출방법보다 더 온화한 조건에서 분리되었다. Alkalin., sodium dodecyl sulfate (pH 12. 45)를 사용하여 chromosomal DNA를 변성시킨 뒤
The large plasmid (about 180 megadaltons) was isolated from the aquatic strain of Pseudomonas which was found to degrade salicylate. It was found that the plasmid could be isolated under gentle conditions in comparison with other methods. The yield of covalently closed circular DNA was enganced by heat treatment at