Effect of Sodium deoxycholate and Sodium dodecy sulfate on Phospholipid Composition and Phospholiases of Rhizopus oryzae

Rhizopus oryzae의 인지질과 그 분해효소에 미치는 계면활성제의 영향

Effect of sodium deoxycholate and sodium dodecyl sulfate on Rhizopus oryzae were investigated. Morphological change was obtained by supplement of these surfactants into culture media during the sumerged culture. In accordance with morphological changes, composition of phospholipid was changed. In case of surfactant-free culture, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine were measured more than 95% of total phospholipid. But cardiolipin and phosphatidylinositol were conspicuously increased by treatment of both sufactants. Presence of phospolipase A, C, and D were detected from mycelium. Phospholipase A and D were activated by supplement of sodium deoxycholate and C was activated by sodium dodecyl sulfate. These results were interpreted in respect of polymorphism of phospholipid and membrane stability against solubilization effect of surfactants.

Rhizopus oryzae 균사체 내의 인지질 구성 성분은 6종으로 나타났고 sodium deoxycholate와 sodium dodecyl sulfate를 각각 0.48mM, O.18mM의 낮은 농도로 영양배지에 첨가했을때 성장군략의 형태변화와 아울러 미량 존재하던 cerdiolipin과 phospha tidylinositol 그러고 Iy sophospholipid의 함량이 증가하였다. 또한 phospholipase는 A와 C, 그리고 D가 존재하였으며 A와 D는 sodium deoxycholate에 의하여. C는 sodium dodecyl sulfate에 의하여 활성도 증가를 나타냈다.