Toxicological Research
- Volume 2 Issue 1
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- Pages.1-7
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- 1986
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
DETECTION OF DNA SINGLE-STRAND BREAKS AND UNSCHEDULED DNA SYNTHESIS INDUCED BY PROCARCINOGENS IN PRIMARY CULTURES OF RAT HEPATOCYTES
- Kim, D.H. (Departmenst of Biological Science and Engneering Korea Advanced Institute of Science and Technology) ;
- Kim, Bok-Ryang (Departmenst of Biological Science and Engneering Korea Advanced Institute of Science and Technology) ;
- K. H. Yang (Departmenst of Biological Science and Engneering Korea Advanced Institute of Science and Technology)
- Published : 1986.06.01
Abstract
Procarcinogen induced DNA single-strand breaks and unschduled DNA synthesis were measured in primary rat hepatocytes culture. For DNA single-strand breaks assay, rat liver DNA was prelabeled by injection 3H-thymidine during the peak of DNA synthesis following partial hepatectomy. Hepatocytes were isolated from the rat 2 weeks after surgery by a collagenase perfusion techinique and maintained as monolayers in serum free medium on collagen-coated culture dishes. DNA sigle-strand breaks were measured by the alkaline elution techinique.