한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제10권1호
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- Pages.109-120
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- 1986
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- 1226-5284(pISSN)
Studies on In Vitro Capacitation by Lysolecithin and In Vitro Fertilizing Ability of Ejaculated Rabbit Sperm
- Kim, C.K. (Dept. of Animal Science, Chung-Ang University) ;
- Im, K.S. (Dept. of Animal Science, Seoul National University) ;
- Zheng, X. (Beijing Agricultural University) ;
- Foote, R.H. (Department of Animal Science, Cornell University)
- 발행 : 1986.05.01
초록
This study was conducted to define the effect of addition of lysolecithin (LC) and 20% v/v rabbit serum to sperm preincubation medium on the induction of acrosome reaction (AR) an fertilizing ability in vitro of LG-added sperm. Ejaculated rabbit sperm from New Zealand White buck was washed once by centrifugation, then preincubated for 2 or 4 hrs in a chemically defined medium (DM), DM plus 20% rabbit serum or BSA-free DM plus 20% rabbit serum at 37
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