한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제10권1호
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- Pages.42-48
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- 1986
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- 1226-5284(pISSN)
H-Y항체의 처리가 생쥐수정란의 발달에 미치는 영향
Effect of H-Y Antibody on in vitro Development of Mouse Embryos
초록
These experiments were carried out to develop new techniques identifying XX-bearing embryos prior to implantation by immunological method. Antiserum to histocompatibility-Y(H-Y) antigen was prepared in adult SD(sprague-dawley) female rat by repeated immunization of newbone testis supernatant from males of the same strain. ELISA test was used to identify the H-Y antibody of antiserum. Total 124 mouse embryos (8-cell stage) were treated with H-Y antiserum and complement in BSA free Ho, pp. and Pitt's medium and cultured under the gas phase of 5% CO2 in air at 37
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