한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)
- 제15권4호
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- Pages.40-46
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- 1986
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- 1226-3311(pISSN)
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- 2288-5978(eISSN)
beta-Galaetosidase의 고정화 및 응용에 관한 연구 제2보: Aspergillus niger CAD 1의 효소 교정화 조건 및 고정화 효소의 성질
Studies on immobilization and application of beta-galactosidase II. Preparation and properties of the immobilized enzyme from Aspergillus niger CAD 1
- 이용규 (전남대학교 농과대학 낙농학과) ;
- 전순배 (전남대학교 자연과학대학 생물학과) ;
- 최원기 (전남대학교 자연과학대학 화학과) ;
-
정기철
(전남대학교 농과대학 낙농학과) ;
- 배석 (전남대학교 자연과학대학 생물학과) ;
- 김관천 (전남대학교 자연과학대학 생물학과)
- Lee, Yong-Kyu (Department of Dairy Science, College of Agriculture, Chonnam National University) ;
- Cuun, Soon-Bae (Department of Biology, College of Natural Sciences, Chonnam National University) ;
- Choi, Won-Ki (Department of Chemistry, College Natural Sciences, Chonnam National University) ;
-
Chung, Ki-Chul
(Department of Dairy Science, College of Agriculture, Chonnam National University) ;
- Bae, Suk (Department of Biology, College of Natural Sciences, Chonnam National University) ;
- Kim, Kwan-Chun (Department of Biology, College of Natural Sciences, Chonnam National University)
- 발행 : 1986.12.30
초록
제1보에서 보고한 Aspergillus niger CAD 1의 beta-galactosidase를 cellulose triacetate를 담체로 하여 고정화조건을 검토하였던바, 13%(v/v)의 정제된 효소와 10%(w/v)의 cellulose triacetate를 30분간, 150stroke/min으로 진탕하면서 포괄시킨 고정화 효소의 활성이 가장 좋았다. 고정화 효소의 최적온도는
The beta-galactosidase of Aspergillus niger CAD 1 described in previous report was immobilized to cellulose triacetate. The optimal conditions for immobilization of the enzyme were obtained when 13%(v/v) of the soluble enzyme (349 unit/ml) was entrapped in fiber prepared from 10%(w/v) cellulose triacetate in methylene chloride. The optimal temperature and pH for the activity of the immobilized enzyme were
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