Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Quantitative Determination of 4-methylthio-3-butenyl isothiocyanate in Radish Root by RP-HPLC

RP-HPLC법에 의한 무우의 4-methylthio-3-butenyl isothiocyanate의 정량

  • Kim, Mee-Ree (Department of Food & Nutrition, College of Science, Chungnam National University) ;
  • Rhee, Hei-Soo (Department of Food & Nutrition, College of Home Economics Seoul National University)
  • 김미리 (충남대학교 식품영양학과) ;
  • 이혜수 (서울대학교 식품영양학과)
  • Published : 1986.12.01
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Abstract

RP-HPLC (Reverse phase-high performance liquid chromatography) assay which was introduced to measure quantitatively the amount of 4-methylthio-3-butenyl isothiocyanate in the radish root, proved to be convenient, accurate and reproducible, showing a good linearity between 10 n moles/ml and 120 n moles/ml (r=0.9997). The results of this assay showed that while the isohtiocyanate was hydrolyzed slowly in the basic medium, it decomposed rapidly in the acidic aqueous medium. On the other hand, the isothiocyanate was relatively stable in the organic solvent (65% acetonitrile). Also, it was found that intact root of Korean radish contain 210-420 ${\mu}moles/100g$, based on the measurement with radish homogenate (pH 8.5, 1 min).

RP-HPLC방법에 의해 무우 속에 존재하는 MTBI의 함량을 정량적으로 측정 해 본 결과 10n moles/ml와 120 nmoles/ml사이에서 좋은 직선적인 상관관계(r=0.9997)를 나타내었으며, 편리하고 정확(오차 5%내외)하며 재현성(recovery 99%)이 높았다. 또, MTBI는 알카리용액 중에서는 서서히 분해되었으나 산성용액 중에서는 매우 빠르게 분해되었으며, 이 결과는 231nm에서 UV흡광도가 서서히 감소하는 결과와도 일치하였다. 한편, MTBI는 유기용매(65% acetenitrile)에서 비교적 안정하였다. 또한. 한국산 생무우 중에 함유된 MTBI는 최적추출조건을 PH8.5에서 1분내에 분석하였을때 $210\sim420{\mu}moles/100g$ 정도이었다.

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