The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Induction of Extracellular Polyphenol Oxidase from Two White-rot Fungi

목재(木材) 부후균(腐朽菌)의 목질소(木質素) 분해효소(分解酵素) 유도(誘導)에 관하여

  • Kim, Kyu-Jung (Department of Biology, Kang-Reung National University) ;
  • Shin, Kwang-Soo (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
  • Hong, Soon-Woo (Department of Microbiology, College of Natural Sciences, Seoul National University)
  • 김규중 (강릉대학 생물학과) ;
  • 신광수 (서울대학교 자연과학대학 미생물학과) ;
  • 홍순우 (서울대학교 자연과학대학 미생물학과)
  • Published : 1986.03.30
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Abstract

Among the representative phenolic compounds in relation to lignin derivatives and protein synthesis inhibitors, the most effective inducer for the extracellular polyphenol oxidase (PO) of Lentinus edodes JA01 was gallic acid and ferulic acid for Pleurotus ostreatus. Optimum concentration of these inducers was 2.0 mM and 1.0 mM, respectively. Addition of gallic acid after two days culture had the best effect on production of PO enzyme of L. edodes JA01 and for P. ostreatus, and addition of ferulic acid after three days culture had the best effect. Also, in case of L. edodes JA01, polyphenol oxidase activity was parallel to growth curve, whereas the maximum enzyme activity of P. ostreatus was shown at exponential growth phase and declined thereafter.

리그닌과 관련된 대표적인 페놀화합물인 ferulic acid, vanillic acid 및 gallic acid와 단백질합성저해항생제인 cycloheximide와 puromycine 중에서 Lentinus edodes JA01 경우는 gallic acid가 Pleurotus ostreatus는 ferulic acid가 각각 가장 효과적인 inducer였다. gallic acid는 2.0mM에서, ferulic acid는 1.0mM에서 induction이 가장 잘 되었다. Inducer의 처리시기는 접종후 L. edodes는 2일후에, 그리고 P. ostreatus는 3일후에 최고의 활성을 나타내었다. 또한 polyphenol oxidase activity는 균주의 생장곡선에 대체적으로 비례하여 증가하였으나, P. ostreatus 경우는 대수기에서 최고의 활성을 나타내고 그 이후 감소하였다.

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