The Korean Journal of Mycology (한국균학회지)
- Volume 14 Issue 1
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- Pages.9-15
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- 1986
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Genetic Analysis of the Life Cycle in Interspecific Hybrids of Pleurotus ostreatus and Pleurotus florida Following Protoplast Fusion
원형질 체융합에 의한 느타리버섯과 사철느타리버섯 체세포 잡종의 유전 분석
- Yoo, Young-Bok (Applied Mycology and Mushrooms Division, Institute of Agricultural Sciences) ;
- You, Chang-Hyun (Applied Mycology and Mushrooms Division, Institute of Agricultural Sciences) ;
- Park, Yong-Hwan (Applied Mycology and Mushrooms Division, Institute of Agricultural Sciences) ;
- Peberdy, John F. (Department of Botany, University of Nottingham)
- Published : 1986.03.30
Abstract
Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
느타리버섯과 사철느타리버섯의 영양요구성 균주를 원형질체융합에 의하여 이종간융합균주를 선발하였다. 이 체세포잡종을 재배하여 자실체에서 담자포자를 분리하여 life cycle에 대한 유전분석을 한 결과를 요약하면 다음과 같다. 1.
Keywords
- Pleurotus ostreatus;
- Pleurotus florida;
- Protoplast fusion;
- Genetic analysis;
- Recombination;
- Clamp connection