한국응용곤충학회지 (Korean journal of applied entomology)
- 제25권2호
- /
- Pages.71-76
- /
- 1986
- /
- 1225-0171(pISSN)
- /
- 2287-545X(eISSN)
Bacillus thuringiensis serovar. thuringiensis의 내독소 생산조건
Cultural Conditions for Endotoxin Production by Bacillus thuringiensis serovar. thuringiensis
-
Lee, Hyung-Hoan
(Institute for Genetic Engineering and Department of Biology, Kon Kuk University) ;
- Lee, Hee-Moo
- 발행 : 1986.07.30
초록
Bacillus thuringiensis var. thuringiensis H1균을 4종류의 배지에서 배지 조성 및 pH 조건에 따라서 성장 양상과 아포와 내독소 결정체 생산을 조사했다. 1. 4개의 M-배지중 pH 9인 M-3배지에서 BTT의 증식량과 내독소 결정체 및 아포 생산수가 가장 높았다. 150ml배양에서 BTT의 생산량은 3.218g이었고, 생존 아포수는 ml당
Bacillus thuringiensis var. thuringiensis H1 (BTT) strain was cultured in the 4 different fermentation media and then measured their growths and the productions of endotoxin crystals from the culture media. Out of the 4 media, the productions of the endotoxin crystals and spores were maximal in the pH9-M-3 medium. The wet weight of BTT cells grown in the 150ml culture was approximately 3.218g and the number of the viable spores was
키워드