한국식물병리학회지 (Korean Journal Plant Pathology)
- 제1권1호
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- Pages.12-16
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- 1985
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- 0256-8608(pISSN)
형광현미경적 기법에 의한 대추나무, 뽕나무 및 일일초의 마이코플라스마 감염진단
Fluorescence Microscopic Diagnosis of Mycoplasma Infections in Jujube, Mulberry and Periwinckle Plants
- Bak Won Chull (Department of Agricultural Biology, College of Agriculture, Seoul National University) ;
- La Yong Joon (Department of Agricultural Biology, College of Agriculture, Seoul National University)
- 발행 : 1985.01.01
초록
마이코플라스마에 감염된 대추나무, 뽕나무 및 일일초의 조직절편을 형광염색소인 DAPI(4'-6-diamidino-2-phenylindole
Attempts were made to evaluate the efficacy of three fluorochromes, i.e., DAPI (4'-6-diamidino-2-phenylin-dole-2HCl), aniline blue and quinacrine(quinacrine mustard dihydrochloride) for the detection of mycoplasma infections in jujube (Zizyphus jujuba), mulberry (Mows alba) trees and periwinckle (Catharanthus roseus) plant by fluorescence microscopy. Stem sections from these plants infected with mycoplasma-like organisms (MLO) produced distinct fluorescence in the phloem when stained with DAPI, aniline blue or quinacrine, while fluorescence was absent in the healthy plants. The use of these fluorochromes provided simple and efficient techniques for the diagnosis of MLO infections. IOf the three fluorochromes tested, DAPI was found to be most efficient.