한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제13권1호
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- Pages.51-57
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- 1985
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Bacillus thuringiensis의 내독소(內毒素)와 플라스미드의 분리에 관한 연구
Studies on the Isolation of δ-Endotoxin and Plasmids in Bacillus thuringiensis
- Oh, Sang Soo ;
- Lee, Hyung Hoan (Molecular Microbiology Laboratory, Department of Biology Konkuk University)
- 투고 : 1985.01.31
- 발행 : 1985.03.01
초록
Bacillus thuringiensis subspecies중 HD-1, HD-9, HD-73과 finitimus의 내독소와 플라스미드분리에 대하여 얻은 실험결과는 다음과 같다. 1. 밀도구배에 의한
Delta-endotoxin in Bacillus thuringiensis var. finitimus, HD-1, HD-9 and HD-73 strains were isolated by NaBr, CsCl and Renografin density gradients. The purity of the toxin was about 98%. The purified o-endotoxin was analyzed by SDS-PAGE, electron microscopic observation and bioassay. According to SDS-PAGE, the molecular weight of subunits of the o-endotoxin were about 66,000 and 130,000 daltons. The shapes of the crystal toxin observed by TEM except finitimus strain were bipyramidal. When the purified endotoxin was bioassayed against tobacco horn worm, the entomocidal activities (
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