Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 13 Issue 1
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- Pages.27-31
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- 1985
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Study of Interferon Assay by HEp-2 : VSV system
HEp-2 : VSV system을 이용한 인터페론 역가측정 연구
- Jung, In Whoan (R & D center, Cheil Sugar Co.) ;
- Chang, Uk (R & D center, Cheil Sugar Co.) ;
- Kim, Hyun Su (R & D center, Cheil Sugar Co.) ;
- Bai, Jong Chan (R & D center, Cheil Sugar Co.) ;
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Lee, Won Yung
(Department of Microbiology, College of Medicine, Yonsei University)
- 정인환 (제일제당(주) 종합연구소) ;
- 장욱 (제일제당(주) 종합연구소) ;
- 김현수 (제일제당(주) 종합연구소) ;
- 배종찬 (제일제당(주) 종합연구소) ;
-
이원영
(연세대학교 의과대학미생물학교실)
- Received : 1985.01.29
- Published : 1985.03.01
Abstract
A rapid assay for interferon based on reduction of cytopathic effect was developed with HEp-2 and Vesicular Stomatitis Virus. The number of manipulations and the lengths of the various incubation steps were reduced to minimum. The assay is simple to perform and can be completed with 22-24 hr. Moreover, it was precise method than CPE-reading method.
WISH, FL 및 HEp-2 세포주중 분석용 세포주를 선정하기 위하여 VSV에 대한 sensitivity와 세포성장능을 조사하였다. 그 결과 HEp-2 세포주가 가장 유리한 것으로 판별되었으며, 이 세포주를 이용하여 초기 세포농도
Keywords