The Journal of the Korean Society for Microbiology (대한미생물학회지)
- Volume 20 Issue 1
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- Pages.91-102
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- 1985
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- 0253-3162(pISSN)
Methods for Coating the Killed Whole Cell Antigens of Salmonella typhi in Enzyme-linked Immunosorbent Assay
효소면역측정법을 위한 장티푸스 균체항원의 부착방법
- Kim, Youn-Won (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
- Hwang, Eung-Soo (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
- Kook, Yoon-Hoh (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
- Choi, Kang-Won (Department of Internal Medicine, College of Medicine, Seoul National University) ;
- Kim, Ik-Sang (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
- Cha, Chang-Yong (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
- Lee, Seung-Hoon (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University)
- 김윤원 (서울대학교 의과대학 미생물학교실.암연구소) ;
- 황응수 (서울대학교 의과대학 미생물학교실.암연구소) ;
- 국윤호 (서울대학교 의과대학 미생물학교실.암연구소) ;
- 최강원 (서울대학교 의과대학 내과학교실) ;
- 김익상 (서울대학교 의과대학 미생물학교실.암연구소) ;
- 차창용 (서울대학교 의과대학 미생물학교실.암연구소) ;
- 이승훈 (서울대학교 의과대학 미생물학교실.암연구소)
- Published : 1985.12.31
Abstract
The advantages of enzyme-linked immunosorbent assay(ELISA) are its senstivity and simplicity in detecting IgG, IgM and IgA antibody. To apply ELISA to diagnosis of typhoid fever, antigen such as lipopolysaccharide of Salmonella typhi or killed whole cell must be coated on solid phase. It is easy to coat lipopolysaccharide on ELISA plate but troublesome to purify it. As it is easy to obtain the killed whole cells, the development of the appropriate method by which those antigens of S. typhi are optimally coated on solid phase is needed. To establish the appropriate method, carbonate buffer, methanol or poly-L-lysine was applied as binding substance on polystyrene or polyvinylchloride plate as solid phase when the killed whole cell antigens of S. typhi varided as follows:
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