$^{99m}Tc-Liposomes$과 킬레이트제 표지반응의 효과

Investigation of Liposomes Labelled with Tc-99m Added EDTA, DTPA and NTA for Tumor Seeker

  • 류용운 (원자력병원 핵의학연구실) ;
  • 김장휘 (원자력병원 핵의학연구실) ;
  • 강태웅 (원자력병원 핵의학연구실) ;
  • 유주현 (연세대학교 식품공학과)
  • Ryu, Yong-Wun (Nuclear Medicine Laboratory, Cancer Research Hospital) ;
  • Kim, Jang-Hwi (Nuclear Medicine Laboratory, Cancer Research Hospital) ;
  • Kang, Tae-Woong (Nuclear Medicine Laboratory, Cancer Research Hospital) ;
  • Yu, Ju-Hyun (Department of Food Engineering, Yonsei University)
  • 발행 : 1985.05.20

초록

Using chelating agents such as Nitrilotriaceticacid(NTA), ticacid(DTPA) and Ethylenediaminenitrilotetraceticacid(EDTA), with TC-99m were determined in vitro and in vivo. Methods.of separation and determination of TC-99m-liposomes added chelating agents were practiced by thin layer chromatogram scan and gel filtration. Biodistributions of Tc-99m-liposomes in normal and sarcoma 180 cells bearing mice were observed. The results were as follows: 1) Maximum amount of $Sn^{+2}$ to reduction from pertechnetium$(10\sim20{\mu}ci)$ by adding 0, 1, 10 and $100{\mu}g$ of $SnCl_2$ in 0.2 ml of oxygen free water was $10{\mu}g$. 2) The large amounts of $SnCl_2$ were not changed but the small amounts of $SnCl_2$ were much changed by labeling with TC-99m to add chelating agents. EDTA in small amounts of $SnCl_2$ were reduced more strongly than DTPA or NTA. Using a hydrophilic chelate, DTPA, the uptake of liposomes could not accumulated in liver and spleen by a lipophilic chelate NTA were significant in vivo. 3) Uptake by tumor was achived 1.14% of injected dose per gram tissue and tumor to organ ratios were measured in low with TC-99m-NTA-liposomes(+).

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