The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
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Thyroid Stimulating Immunoglobulin Bioassay Using Cultured Human Thyroid Cells; A Simplified Micromethod

갑상선질환에서 갑상선 자극면역글로불린측정의 의의에 관한 연구 -Micro법 갑상선세포배양에 의한 측정의 기본적 검토-

  • Lee, Myung-Chul (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Chung, June-Key (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Cho, Bo-Youn (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Mun-Ho (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Ahn, Il-Min (Department of Internal Medicine, College of Medicine, Korea University) ;
  • Ahn, Hee-Kwon (Department of Internal Medicine, College of Medicine, Korea University)
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 조보연 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 이문호 (서울대학교 의과대학 내과학교실) ;
  • 안일민 (고려대학교 의과대학 내과학교실) ;
  • 안희권 (고려대학교 의과대학 내과학교실)
  • Published : 1985.05.20
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Abstract

The activation of adenylate cyclase of human thyrocytes in primary cell culture and the release of c-AMP into the medium are used to detect b-TSH and TSAb in sera of patients with autoimmune thyroid disease. Sera of patients are used directly as a part of cell culture without immunoglobulin precipitation. In the above TSI bioassay, TSAb pooled serum show c-AMP concentration between that of 1mU/ml and 10 mU/ml b-TSH but normal control pooled serum doesn't show any detectable c-AMP response. Ninety fiye percent of untreated Graves' patients shows TSAb activity above normal range, 20% of Hashimoto's and 36% of euthyroid Graves' patients show detectable TSAb activity.

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