Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Factors Affecting Protoplast Formation of Yeast

효모의 원형질체 형성조건

  • Kim, Young-Ho (Department of Microbiology, Kyungpook National University) ;
  • Seu, Jung-Hwn (Department of Microbiology, Kyungpook National University)
  • Published : 1985.12.01
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Abstract

As an essential previous step towards the development of cell fusion to breed a new brewing yearst strain, several factors predicted to affect the protoplast formation of S. cerevisiae, C. tropicalis and E. fibuligera were investigated in order to obtain the protoplasts in high yields. The optimum pH and temperature for the protoplas formation were 7.5 and 35$^{\circ}C$, respectively. Pretreatment of the yeast cells with 2-mercaptoethanol stimulated the protoplast formation and 50mM of the reagent was found as effective. Among several osmotic stabilizers tested for their effect on protoplas formation, 0.6M KCI was comparatively favorable.

이속 효모간 원형질체 융합에 의해 새로운 알콜발효 균주를 개발하기 위한 전단계로서, Arthro-bacter luteus에서 얻은 zymolyase 5,000을 세포벽 분해효소로 사용하여 전분분해 효모인 C. tropicalis, E. fibuligera 및 알콜발효 효모인 S. cerevisiae 등의 원형질체 형성조건에 대하여 검토하였다. 원형질체 형성의 최적 pH와 온도는 각각 pH 7.5와 35$^{\circ}C$였다. 또한 원형질체 형성에 대한 2-mercapto-ethanol의 효과는 50mM농도에서 가장 좋았으며 삼투압 안정제로는 0.6M KCl이 적당하였다.

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