Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Citrate Production by Sexually Compatible Strains of Saccharomycopsis lipolytica

Saccharomycopsis lipolytica의 성적 화합성 균체에 의한 시트르산 생산

  • Gum, Cho-Seok (Dept. of Food Science and Technology, Chung-Ang University) ;
  • Masayoshi Matsuoka (Department of Fermentation Technology, Faculty of Engineering, Osaka University) ;
  • Shuichi Aiba (Department of Fermentation Technology, Faculty of Engineering, Osaka University)
  • Published : 1985.09.01
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Abstract

Sexually compatible heterothallic haploids and diploids therefrom of Saccharomycopsis lipolytica were compared with respect to production. Diploids constructed through mating were confirmed by random spore analysis, whereas those constructed through protoplast fusion were confirmed by haploidization. ATCC 44601 and IFO 1209 produced larger amount of citrate and isocitrate than IFO 1550 and IFO 1551. A mode of citrate production by diploids was intermediate of the parental haploid strains. The specific activities of citrate synthase and isocitrate lyase in IFO 1550 and IFO 1551 were higher than those in ATCC 44601 and IFO 1209, indicating little correlation between citrate production and specific activities of these enzymes.

성적 화합성 heterothallic 2부체와 1부체인 Saccharomycopsis lipolytica에서 시트르산 생산성을 비교하였다. 교잡과 세포융합에 의한 2부체는 random spore 분석과 단상화에 의하여 확인되었다. ATCC 44601과 IFO 1209 균주는 IFO 1550와 IFO 1551 균주보다 시트르산과 이소시트르산을 많이 생산하였다. 2부체에 의한 시트르산 생산양식은 양친 1부체 균주의 중간정도이였다. IFO 1550와 IFO 1551 균주의 citrate synthetase와 isocitrate lyase의 비활성은 ATCC 44601과 IFO 1209 균주에서 보다 높았고 이들 효소의 비활성과 시트르산 생산간에는 상관관계가 없었다.

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