Glycine Effect on Spheroplasting and Nodule Bacteroids of Rhizobium Jjaponicum

Rhizobium japonicum원형질체 형성과 근류 bacteroids에 미치는 glycine의 영향

Different spheroplasting methods using glycine were tried to fast and slow-growing R. japonicum. Although one of the fast growers, R-271 showed normal growth in the presence of 4mg/ml glycine, cell morphology and colony forming unit (CFU) were greatly different from the cells of late log phase grown in the medium without glycine. In parallel, R-271 became sensitive to lysozyme after 6hr incubation in medium containing glycine (3.5mg/ml). After 24hr cultivation in glycine $(100{\mu}g/ml)$ medium, one of the slow growers, R-214 was also susceptible to lysozyme action. Spgeroplasting frequency of both strains was over 96% by glycine and lysozyme. Spheroid cell was also found in bacteroids from root nodule and soluble glycine content was relativiely smaller than other amino acids in soybean nodule extracts.

Glycine이 R.japonicum의 spheroplasting에 미치는 영향을 검토하였다. Fast-growing R. japonicum R-271은 4mg/ml의 glycine 함유배지에서 생육이 정상이었으나 세포 형태는 배양시간에 따라 변화하였으며 colony 형성단위는 log-phasedlgn 현저히 감소하였다. R-271은 3.5mg/ml glycine 함유배지에서 6시간 배양후에, slow grower R-214는 0.1mg/ml glycine 함유배지에서 24시간 배양후에 lysozyme의 작용을 받았다. 두 균주 모두 이러한 처리에 의해 96%이상의 spheroplast 형성빈도를 나타내었다. 한편 spheroid-type cell은 대두근의 nodule에서 분리한 bacteroid에서도 관찰되었으며 nodule extract에 존재하는 유리 amino acid 중 glycine 은 다른 amino acid에 비해 소량 존재하고 있었다.