Coryne형 제균의 원형질체 융합빈도 향상

Frequency improvement of protoplast fusion in coryneform bacteria

  • 김종헌 (고려대학교 농과대학 농화학과) ;
  • 임번삼 (고려대학교 농과대학 농화학과) ;
  • 이세영 (고려대학교 농과대학 농화학과) ;
  • 전문진 (고려대학교 농과대학 농화학과)
  • 발행 : 1985.09.01

초록

For frequency improvement of protoplast fusion in Brevibacterium flavum, Brevibacterium lactofermentum lactofermentum and Corynebacterium glutamicum, the effect of plasma expanders on fusion and cell wall regeneration, compatison between direct and two-step selection method, tendency of fusion frequency according to pH of fusion fluid and polyethylene glycol concentration were examined. By addition of 3% polyvinyl pyrrolidone to cell wall regeneration medium, regeneration frequencies were expressed 23 (Brevibacterium lactofermentum), 10.4 (Brevibacterium flavum) and 2.7 (Corynebacterium glutamicum) times higher than those of none polyvinyl pyrrolidone medium respectively.

키워드