Protein Patterns of Blood Plasma in Pregnant Women by SDS/polyacrylamide Gel Electrophoresis

SDS/polyacrylamide Gel 電氣泳動에 依한 妊娠한 女子 血漿蛋白質의 패턴

The plasma protein patterns of non-pregnant women, pregnant women, and normal male individuals were analyzed by SDS/polyacrylamide gel electrophresis. When the protein patterns of plasma of normal male individuals ranging from 10, 000 to 110, 000 daltons in molecular weights are compared to non-pregnant women, their protein patterns were the same. In this study, when the plasma of non-pregnant women are compared to pregnant women, no bands were occurred newly, but the quantity of some protein bands were increased or decreased during the pregnant periods. According to the results of measuring the molecular weights of the characteristic protein patterns, which are increasing or decreasing during the pregnancy as compared to the non-pregnant women, it was observed that the proteins over 76, 000 daltons in molecular weights were concerned in the facts mentioned above. That is, the protein of 86, 000 dalton in molecular weight was not increased in quantity until the second trimester of pregnancy, but was increased in the third trimester of pregnancy. The proteins of 91, 000-105, 000 daltons in molecular weights were gradually increased in accordance with the periods of pregnancy. On the contrary, the protein of 94, 000 dalton was rather decreased by the second trimester of pregnancy, but increased in the third trimester of pregancy. And the band of 99, 000 dalton was not changed in quantity significantly until the first trimester of pregnancy, but increased continuously from the second trimester of pregnancy to the third trimester of pregnancy. We tentatively suggest that the stages (the first, the second, and the third trimester) of pregnancy can be identified by the study on the protein patterns of the specific bands in the blood plasma of pregnant women.

非姙娠 女子, 姙娠된 女子 및 正常인 男子의 血漿蛋白質을 SDS/polyacrylamide 젤 電氣泳動으로 分析하였다. 分子量 10,000에서 110,000 dalton 사이의 正常인 男子와 非姙娠 女子의 血漿蛋白質의 패턴은 同一하였다. 本 硏究에서 非姙娠 女子와 姙娠된 女子의 血漿蛋白質을 比較하였을 때 새로운 band들이 나타나지는 않았지만 어떤 蛋白質 band들은 姙娠의 初期, 中期 및 末期에 따라서 量的으로 增加 EH는 減少되었다. 各 band들의 分子量을 測定한 結果 分子量 76,000 dalton 以上의 蛋白質들이 姙娠中 增加 또는 減少되어 짐을 알 수 있었다. 즉 分子量 86,00 dalton의 蛋白質은 姙娠 中期 까지는 增加되지 않았으나 姙娠 末期에는 增加되었다. 分子量 91,000-105,000 dalton 사이의 蛋白質들은 姙娠의 期間이 길어짐에 따라 대체로 量이 增加되었다. 反面에 分子量 94,000 dalton의 蛋白質은 姙娠 中期 까지는 量이 若干 減少 되었으나 末期에는 오히려 增加되었다. 그리고 分子量 99,000 dalton의 蛋白質은 姙娠 初期까지는 變化가 없었으나 中期에서 末期로 감에 따라 漸次的으로 增加되었다. 그러므로 우리들은 姙娠된 女子 血漿의 蛋白質 패턴을 調査함으로써 姙娠의 期間을 알 수 있음을 提示하는 바이다.