SDS Polyacrylamide Gel 電氣泳動에 依한 斧足綱數種의 蛋白質패턴의 比較

The Comparison of Protein Patterns of Several Species in Bivalvia by SDS Polyacrylamide Gel Electrophoresis

斧足綱 數種의 蛋白質을 電氣泳動한 結果, 새꼬막과 피조개의 血漿의 蛋白質 패턴은 低分子 蛋白質에서 染色되는 强度가 약간 差異가 있으나 거의 同一하였고, 全血球의 蛋白質 패턴의 差異는 17,800 dalton의 band에서 나타났다. 그렇지만 위의 두 種의 筋內의 단백질 패턴은 거의 비슷해서 10,000$\\sim$100,000 dalton의 分子量 範圍內에서는 差異를 알아 내기가 매우 어려웠다. 그런데 대합과 비늘백합은 類緣關係가 있는 蛋白質 패턴이 많이 나타났으며 재첩은 대합이나 비늘백합과 같은 이들 두 種과 대칭이의 中間 程度에 位置하는 蛋白質 패턴을 나타내고 있었다. 본 연구에서는 Bivalvia가 함께 다 갖는 6개의 蛋白質 band들과, 꼬막과 새꼬막에서만 나타나는 4개의 特有한 蛋白質 band가 存在함이 發見되었으며 Eulamellibranchia目의 4종에 있어서 다 나타나는 2개의 protein band들 과, 대합과 비늘백합에서만 나타나는 23,000 dalton의 特有한 band도 發見되었다. 그리고 各 種마다 가지는 特有한 蛋白質 패턴의 分子量을 測定하여 比較하였다.

SDS-polyacrylamide gel electrophoresis for the proteins obtained from the plasma, Scapharca subcrenata and suapharca broughtonii, and for the proteins of muscles of several species in Bivalvia was performed. The protein patterns of plasma showed little difference between S. subcrenata and S. broughtonii in lower molecular weight weight proteins. However, the protein patterns of muscles of other species, which wre used in this study, were more shown in the lower molecular weights than the higher molecular weights in difference. Thus it is thought be an interesting fact. The protein band of blood corpuscles, 17,800 dalton, was not appeared in S. broughtonii, but this band was appeared in S. subcrenata. Henceforth this is the significantly important difference in these two species. But the protein patterns obtained from muscles of the species did not show a difference in a range of molecular weights between 10,000 and 100,000 daltons. Meanwhile, several protein bands obtained from Meretrix lusoria were similar to those of Mercenaria stimpsona. Hence, in this study, 6 protein bands which exist all species in Bivalvia and 4 characteristic protein bands in S. subcrenata and S. broughtoniionly, were investigated. And in four species of Eulamellibranchia, two protein bands in comon and the characteristic band of 23,000 dalton which is belong to Meretrix lusoria and Mercenaria stimdsona, were found. The molecular weights of the characteristic protein patterns, which are contained in each species, were measured and compared.