Parasites, Hosts and Diseases
- Volume 23 Issue 1
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- Pages.95-101
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- 1985
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Application of Micro-BLISA in Serodiagnosis of Fascioliasis in Cattle
간질증의 효소면역학적 진단
- Lee, Jae-Gu (Department of Veterinary Parasitology, Jeonbug National University) ;
- Baek, Byeong-Geol (Department of Veterinary Parasitology, Jeonbug National University) ;
- Lee, Sang-Bok (Department of Veterinary Parasitology, Jeonbug National University)
- Published : 1985.06.01
Abstract
Fascioliasis in cattle is one of the most common and very serious trematode diseases in Korea. In the present study, the enzyme linked immunosorbent assay (ELISA) was applied in the diagnosis of fascioliasis using antigen of Fasciela hepatica, perokidase of conjugate anti-cattle Is G and orthophenylenediamine as a substrate by micro-method technique of Volley et at. (1976b) and MacLaren (1978) with a slight modification. Results obtained from the present study are as follows. 1. In assay for optimal dilution of stock antigen, the antigen (protein contents; 0. Bmgymz) was diluted from 1150 to 1/600 with carbonate buffer (pH 9.6), and then absorbance values were measured with 1/100 diluted sera. The regression equations between the OD values of ELISA and dilution of antigen were log Y: -0.181-0.00127X in infected sera, and log Y: -0.578-0. 000879X in normal sera. The significantly higher (p<0.05) OD value was observed in the former. 2. In assay for optimal dilution of sera, the sera were diluted from 1125 to 1/400 with in PBSJ Tween 20 (pH 7.4), and absorbance values were measured with 1/200 diluted antigen. The regression equation between the OD values of ELISA and dilution of sera were log Y: -0.1540-0.0007238X in infected sera and log Y: -0.4834-0.00116X in normal sera. The former was higher than the latter (p<0.05). 3. In the 27 cases of negative intradermal test, OD values of the ELISA are
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