Applied Biological Chemistry
- Volume 28 Issue 1
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- Pages.13-18
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- 1985
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Isolation of Uracil and Tryptophan Auxotrophs of Bacillus subtilis and Regeneration of their Protoplasts
Bacillus subtilis의 $Ura^-$ 와 $TrP^-$ 균주(菌株)의 육성(育成)과 Protoplast 조제(調製) 및 Regeneration
- Ha, Il-Ho (Dept. of Food Sci. and Technol., College of Agriculture, Seoul National University) ;
- Lee, Ke-Ho (Dept. of Food Sci. and Technol., College of Agriculture, Seoul National University)
- Published : 1985.03.30
Abstract
To investigate the fusion of bacterial protoplasts, two auxotrophic mutants of Bacillus subtilis were isolated after treatment with nitrosoguanidine. Two auxotrophs were uracil-auxotroph and tryptophan-auxotroph. Back mutation frequencies of
세균의 protopalst 융합을 연구하기 위하여 Bacillus subtilis로부터 두 개의 영양 요구성 균주를 NTG처리에 의해서 분리하였다. 두 개의 영양 요구성 균주는 Uracil 요구성 균주와 Tryptophan 요구성 균주였다.
Keywords