Effects of Germination on Antinutritional Oligosaccharides of Mung Beans

녹두발아시 항영양 과당류의 변화

To examine changes of antinutritional oligosaccharides, particularly raffinose and stachyose, of mung beans during germination, mung beans (Phaseolus aureus) were germinated at $25^{\circ}C$ for 7 days after soaking for 15 hr. The 80% ethanol extracts of mung bean sprouts were analyzed by Kieselgel thin-layer chromatography using a solvent system, ethylacetate, acetic acid and water (3:1:1, v/v). The moisture level was increased greatly after soaking followed by stabilization along to the 3rd day of germination. The level was increased slightly on the 4th day and thereafter stabilized. Antinutritional oligosaccharides, raffinose and stachyose was diminished significantly on a thin-layer chromatogricadtly on the 1st and 2nd day of germination, and disappeared almost thereafter.

녹두발아중 항영양 과당류의 변화를 알아보기 위하여 실온에서 15시간 침지시킨 녹두(Phaseolus aureus)를 $25^{\circ}C$에서 7일간 발아시키면서 실리카박층크로마토그라피로 당의 패턴을 분석하였다. 녹두의 수분함량은 15시간 침지이후 발아 3일까지 65%로 유지되다가 4일째부터는 82%로 거의 안정하였다. 항영양 과당류인 raffinose와 stachyose는 박층크로마토그라피상에서 발아 $1{\sim}2$일째 급격히 감소하여 미량 존재 하다가 3일째부터는 거의 소멸되었다.