굴비의 가공 및 저장중의 N-Nitrosamine에 관한 연구 1. 굴비의 가공 및 저장중 질산염, 아질산염 및 아민류의 변화

In recent years, the occurrence of nitrosamine, which are produced by the interaction of nitrite and secondary amine, in foods has been the subject of considerable interest and controversy. In this experiment, changes in nitrate, nitrite, TMAO, TMA and DMA nitrogen of salted and dried corvenia, which were incorporated with sodium benzoate, ascorbic acid, cysteine and tetracycline in salt, during processing and storage were analyzed. Levels of nitrate nitrogen was decreased while those of nitrite was increased in salted and dried products during processing and storage, but sample was incorporated with ascorbic acid, cysteine and sodium benzoate in salt inhibited the reduction of nitrate to nitrite while with tetracycline in salt accelerated this reduction in salted and dried products during processing and storage. Contents of TMAO nitrogen in all salted and dried products as well as in the control was decreased, but was increased during storage, while TMA nitrogen was increased in salted and dried products during processing and storage. Contents of DMA nitrogen was increased in all salted and dried products during processing and storage, DMA contents of raw in the control was increased to more than 11.6 times after storage for 30 days, but sample was incorporated with ascorbic acid, cysteine and sodium benzoate in salt inhibited the production of DMA nitrogen while with tetracycline in salt accelerated in salted and dried products during processing and storage.

굴비의 가공 및 저장중 발암성물질로 알려진 nitrosamine의 전구물질인 아질산염질소 및 DMA 질소의 생성을 억제하기 위하여 염장시 몇몇종의 보존료를 첨가하여 이들의 변화를 분석 검토하였고, 동시에 이들의 생성에 관련이 있다고 생각되는 질산염질소, TMAO 및 TMA 질소의 변화를 실험하였다. 굴비의 가공 및 저장중 질산염질소는 계속해서 감소하였고, 반면에 아질산염질소는 증가하는 경향이었다. 그러나 ASC 구, CYS 구 및 BEN 구는 질산염의 환원을 억제시켰고, TET 구는 오히려 촉진시키는 결과였다. TMAO 질소는 염장 및 천일건조중 감소하다가 저장중 약간 증가하였으나 TMA 질소는 가공 및 저장 공정중 계속해서 증가하였다. 보존료의 첨가가 TMA 질소의 생성은 크게 억제시키지 못하였다. DMA 질소는 굴비의 가공 및 저장공정중 계속해서 증가하여 대조구의 경우 저장 7일후에는 생시료에 비해 3.2배, 건조 20일후에는 3.6배, 저장 30일 후에는 11.6배에 달하였다. ASC 구, CYS 구 및 BEN 구에서는 DMA 질소의 생성을 크게 억제하여 대조구에 절반치에 가까웠으나, TET 구에서는 오히려 DMA 질소의 생성을 촉진시키는 결과를 나타내었다.