Abstract
The extracted hagfish (Eptatretus burgeri) flesh lipid was separated into following fractions by column chromatography on Bio-beads SX-2 and Sephadex LH-20 prior to gab chromatographic analysis of their fatty acid compositions: polar lipid, triglyceride and free fatty acid. The major fatty acids of total lipid and triglyceride in hagfish were $C_{16:0},\;C_{16:1},\;and\;C_{18:1}$. The ratio of $C_{18:0}/C_{18:1}$ in the total lipid and triglyceride of hagfish was 0.1. The polar lipid of the hagfish muscle was mainly composed of phosphatidyl choline ($65.5\%$) and phosphatidyl ethanolamine ($28.0\%$). The triglyceride obtained was fractionated into four fractions by HPLC on the basis of partition numbers. Both the fatty acid composition and triglyceride composition on the basis of the total carbon number in the acyl chains of the triglyceride were analysed by the GLC. From the information obtained on triglyceride compositions based on the total carbon number by GLC and the partition number by HPLC and fatty acid composition by GLC, the combination of fatty acid in each triglycerides was estimated. A computer was used for estimation of the fatty acid combination in the triglyceride because hagfish lipid triglyceride was composed of various kinds of fatty acids. Fortyfour kinds of triglyceride were estimated. The major triglycerides in hagfish flesh lipid were found to those of ($1{\times}C_{16:0},\;2{\times}C_{18:1};\;13.5\%$), ($1{\times}C_{16:0},\;1{\times}C_{18:0},\;1{\times}C_{18:1};\;7.2\%$), ($1{\times}C_{16:1},\;2{\times}C_{18:1};\;5.4\%$), ($2{\times}C_{16:0},\;1{\times}C_{22:5};\;5.2\%$), ($1{\times}C_{14:0},\;2{\times}C_{18:1};\;4.5\%$), ($2{\times}C_{18:1},\;1{\times}C_{22:5};\;3.6\%$), ($1{\times}C_{14:0},\;1{\times}C_{18:0},\;1{\times}C_{18:1};\;2.7\%$) and ($1{\times}C_{14:0},\;1{\times}C_{16:0},\;1{\times}C_{18:2};\;2.2\%$).
먹장어근육지질을 Biobeads SX-2 및 Sephadex LH-20을 이용한 칼럼크로마토그래피에 걸어 트리글리세리드(TG)획분을 분획하고 이 TG획분을 HPLC에 걸어 partition number별로 분획하였다. 그리고 partition number에 따른 TG조성, 총탄소수에 따른 TG조성 및 지방산조성을 분석한 자료를 computer처리하여 TG 구성지방산조합을 추정하였다. 총지질의 경우 포화산 $34.3\%$, monoene산 $43.1\%$, polyene산 $22.7\%$, 중요지방산은 $C_{16:0}(16.6\%),\;C_{16:1}(12.5\%)$ 및 $C_{18:1}(28.0\%)$였고, 극성지질은 포화산 $31.8\%$, monoene산 $30.1\%$, polyene산 $38.2\%$, 중요지방산은 $C_{16:0}(15.0\%),\;C_{18:0}(10.8\%),\;C_{18:1}(18.5\%),\;C_{22:5}(16.5\%)$ 및 $C_{22:6}(10.3\%)$, TG는 포화산 $29.8\%$, monoene산 $53.1\%$, polyene산 $17.2\%$, 중요지방산은 $C_{16:0}(17.1\%),\;C_{16:1}(10.1\%)$ 및 $C_{18:1}(38.4\%)$였다. 전반적으로 보면 $C_{18:1}$의 함량이 높은 것이 특징이었으며, $C_{18:0}/C_{18:1}$의 비는 0.1이였다. 극성지질은 phosphatidyl choline($65.5\%$)과 phosphatidyl ethanolamine($28.0\%$)으로 구성되어 있었다. GLC 자료 및 HPLC에서 얻은 partition number를 사용하여 먹장어 지질 TG의 구성지방산조합을 computer를 이용하여 추정한 결과 이중결합을 1개, 2개, 3개, 4개, 5개, 6개, 7개 가지고, 총탄소수는 46에서 58의 범위내에 분포하며, $0.1\%$ 이상 함유될 가능성이 있는 TG는 44종류로 추정되었으며 그 합계는 69.75였고, 중요한 TG는 ($1{\times}C_{16:0},\;2{\times}C_{18:1};\;13.5\%$), ($1{\times}C_{16:0},\;1{\times}C_{18:0},\;1{\times}C_{18:1};\;7.2\%$), ($1{\times}C_{16:1},\;2{\times}C_{18:1};\;5.4\%$), ($2{\times}C_{16:0},\;1{\times}C_{22:5};\;5.2\%$), ($1{\times}C_{14:0},\;2{\times}C_{18:1};\;4.5\%$), ($2{\times}C_{18:1},\;1{\times}C_{22:5};\;3.6\%$), ($1{\times}C_{14:0},\;1{\times}C_{18:0},\;1{\times}C_{18:1};\;2.7\%$) 및 ($1{\times}C_{14:0},\;1{\times}C_{16:0},\;1{\times}C_{18:2};\;2.2\%$)등이었다. 이들 TG중 $C_{18:1}$을 1분자이상 함유하는 것의 조함비의 합계는 $52.4\%$, 2분자이상 함유하는 것은 $35.9\%$였다.