Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Identification of Responsible Region for the Polymerization of Plasmid pEC-3

Plamid pEC-3의 중합에 필요한 부위의 동정

  • Jang, Sung-Key (Laboratory of Molecular Genetics, Department of Zoology, Seoul National University) ;
  • Lee, Ha-Kyu (Laboratory of Molecular Genetics, Department of Zoology, Seoul National University) ;
  • Rho, Hyune-Mo (Laboratory of Molecular Genetics, Department of Zoology, Seoul National University)
  • 장승기 (서울대학교 자연과학대학 동물학과 분자유전학 연구실) ;
  • 이하규 (서울대학교 자연과학대학 동물학과 분자유전학 연구실) ;
  • 노현모 (서울대학교 자연과학대학 동물학과 분자유전학 연구실)
  • Published : 1984.01.01
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Abstract

In order to find specific acting site of Rec A protein in plasmic polymerization in E. coli, we randomly deleted various part of pEC-3 (a derivative of pBR322) with SI nuclease treatment. Self-ligated plasmids were introduced into E. coli WA802(Rec $A^+$). A number of colonies were analyzed if they contained monomeric or polymeric plasmids by gel electrophoresis. The plasmid (pEC-43), which was deleted the region of tetracycline gene, revealed only monomeric form in Rec $A^+$ E. coli. When two plasmids, pEC-3 and pEC-43, were co-transformed in the same E. coli, the original pEC-3 showed polymerization but pEC-43 revealed monomeric form only. These results suggest that Rec A protein requires the specific site for polymerization.

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