Korean Journal of Microbiology (미생물학회지)
- Volume 22 Issue 3
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- Pages.175-181
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- 1984
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
The protoplast formation, regeneration and fusion of coryneform bacteria
Coryneform bacteria의 原形質體 形成, 再生 및 融合에 관한 硏究
- Shin, Myung-Gyo (Dept. of Agricultural Chemistry, Korea Univ) ;
- Lee, Se-Yong (Dept. of Agricultural Chemistry, Korea Univ) ;
- Lim, Bun-Sam (Dept. of Agricultural Chemistry, Korea Univ) ;
- Chun, Moon-Jin (Dept. of Agricultural Chemistry, Korea Univ)
- Published : 1984.01.01
Abstract
In order to develope a protoplast fusion system for industrial coryneform bacteria, the optimum conditions for the formation and regeneration of progoplast were examined for Brevibacterium flavum and Corynebacterium glutamicum and the protoplast fusion was performed. For the formation of the protoplast of B. flavum and C. glutamicum, the optimum time for penicillin G. treatment to obtain protoplast was mid-exponential growth phase (
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