Korean Journal of Microbiology (미생물학회지)
- Volume 22 Issue 3
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- Pages.143-150
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- 1984
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Induction of Colonial Growth of the Cellulolytic Fungus, Genus Trichoderma
섬유소분해균인 Trichoderma의 群體生長의 유도에 관하여
- Park, Hee-Moon (Dep. of Microbiology, College of Natural Science, Seoul Natural University) ;
- Hong, Soon-Woo (Dep. of Microbiology, College of Natural Science, Seoul Natural University) ;
- Hah, Yung-Chil (Dep. of Microbiology, College of Natural Science, Seoul Natural University)
- Published : 1984.01.01
Abstract
The effect of growth inhibitors on colonial growth of cellulolytic fungus, genus Trichoderma, was investigated to develop a method for the effective screening of various auxotrophs and hypercellulolytic strains. As the results, it was revealed that non-ionic detergents such as Sodium deoxycholate and Triton X-100 were the better ones as a growth inhibitor than Oxgall which has been used to restrict the colony size in genus Trichoderma. Each individual colony remained distinct on minimal plate supplemented with 0.05% Triton X-100 for as long as two or more weeks. The screening of 150 to 200 colonies simultaneous on a single plate was possible in the presence of Triton X-100. The effect of Triton X-100 on simultaneous screening was higher than that of Oxgall by a factor of two.
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