Determination of ${\beta}$-Lactam Antibiotics by Gas-Chromatography with Flame Photometric Detector (II)

GC/FPD를 利用한 ${\beta}$-락탐系 抗生物質의 分析(II)

For gas chromatographic determination with the sulfur-specific flame photometric detector, nine ${\beta}$-lactam antibiotics without ${\alpha}$-amino group were esterified with borontrifluoride-methanol complex and then N-benzoylated with benzoyl chloride. The gas chromatographic separation of these products was successfully carried out on various silicon polymers (OV-1, OV-101, OV-17, OV-225, and QF-1) coated on the acid washed, silanized diatomite. The structure of the esterified and N-benzoylated product was confirmed by mass spectromer.