Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Microprocessor Based Laser Induced Fluorometry I. Development of System and Application to Liquid Chromatography

  • Kim, Ha-Suck (Department of Chemistry, Seoul National University) ;
  • Park, Chan-Seung (Department of Chemistry, Seoul National University) ;
  • Hwang, Eui-Jin (Department of Chemistry, Seoul National University) ;
  • Chol, Q.-Won (Department of Chemistry, Seoul National University)
  • Published : 1984.12.20
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Abstract

An analytical applicability of the fluorescence detection with an optical multichannel analyzer to organic dyes was studied in this work. Continuous acquisition of spectra was possible with the use of a microcomputer. The maximum acquisition rate of a spetrum with 70-point average was about 3 seconds. Floppy discs were used to store data for later use in processing. Laser induced fluorescence detector in High Performance Liquid Chromatography was chosen for an application. Fluorescein below $10^{-15}g$ was detected satisfactorily using this system. With the help of microcomputer, three dimensional chromatograms of time-wavelength-intensity were obtained.

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