Studies on the Effect of Copper Ion on the Lactate Dehydrogenase Isozyme Patterns in Organs of Catfish, Parasilurus asotus

메기 (Parasilurus asotus)의 장기내 젖산수소이탈효소 아이소자임 형에 미치는 동이온의 영향에 관한 연구

The effect of copper ion on the lactate dehydrogenase isozyme patterns in the heart, liver, kidney, skeletal muscle, and ovary of catfish, Parasilurus asotus, was studied by cellulose acetate gel electrophoresis. 1. The LDH-1 and LDH-2 of heart type appeared in the heart muscle of control fish. When the fish were exposed to copper ion, however, the LDH-1, LDH-2, LDH-3, and LDH-4 appeared. The amount of LDH-1 was decreased and those of LDH-2, LDH-3, and LDH-4 were increased. 2. There was one band of LDH-4 in the liver of normal fish. But the amount of LDH-4 was decreased and additional new LDH-5 appeared by exposure to copper ion. 3. There were LDH-1, LDH-2, and LDH-4 in the kidney tissue of both control and experimental groups. The LDH-1 was increased, whereas LDH-2 and LDH-4 were decreased after exposure to copper ion. 4. There was broad band of LDH-5 in the skeletal muscle of the control fish. However, the LDH-4 and LDH-5 with M sub-band appeared by the exposure to copper ion. 5. There was LDH-3 band only in the ovary of control, wheras all five LDH isozymes appeared in the ovary of the fish exposed to copper iion. 6. During the period of exposure to copper ion, the LDH isozyme of heart type which associated with aerobic metabolism was decreased, but the LDH isozyme of muscle type of anaerobic metabolism was increased in most of heart, liver, and skeletal muscle. It seems that these organs are related to some of important functions for anaerobic metabolism during the copper poisoning period.

메기 (Parasilurus asotus)의 심장, 간장, 신장, 골격근 및 난소내의 젖산수소이탈효소 isozyme 형에 미치는 동이온의 영향을 cellulose acetate gel을 이용하여 전기영동법으로 연구하였다. 1. 정상인 메기의 심장에서는 LDH-1과 LDH-2가 나타났으나 동이온 처리구에서는 LDH-1, LDH-2, LDH-3 및 LDH-4가 나타났다. LDH-1의 양은 감소되고 LDH-2, LDH-3 및 LDG-4의 양은 증가되었다. 2. 정상인 메기의 간장에는 LDH-4만 있었으나 동이온 처리구에서는 LDH-4의 양이 감소되고 LDH-5가 새로 나타났다. 3. 신장에서는 대조구와 처리구, 2군에서 LDH-1, LDH-2 및 LDH-4가 나타났는데, 동이온 처리후 LDH-1의 양은 증가되고 LDH-2와 LDH-4는 감소되었다. 4. 대조구의 골격근에는 넓은 band의 LDH-5가 하나 있었으나 처리구에서는 LDH-4와 LDH-5가 나타났는데 특히 LDH-5는 하나의 M형 sub-band를 가지고 나타났다. 5. 정상인 난소에서는 LDH-3 하나만 나타났으나 동이온 처리구에서는 LDH-1에서 LDH-5RK지의 5개 LDH isozyme이 모두 나타났다. 6. 동이온 처리 기간중 심장, 간장 및 골격근에서는 대개 유기대사와 연관된 심장형 LDH isozyme은 감소되었지만 무기대사와 연관된 근육형 LDH isozyme은 증가되었다. 이들 기관은 동중독시의 무기대사의 중요한 기능과 어느 정도 관련이 있는 것으로 생각된다.