한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제8권1호
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- Pages.22-28
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- 1984
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- 1226-5284(pISSN)
Rat 수정란의 동결보존에 있어 동결속도 및 동해방지제에 관한 연구
The Study on the Freezing Methods and the Cryoprotectants for Rat Embryo Preservation
초록
This experiment was carried out to investigate effects of DMSO or ethylene glycol as a cryopotectant and of freezing methods on survival rate of forozen-thawed rat 2-cell embryos by morphological observation. 2-cell embryos were recovered from oviducts of Sprague Dawley females mated with males of same strain on day 2 of pregnancy after inducing superovulation by intrapertioneal injection of PMSG and HCG. In slow freezing and thawing groups, embryos were frozen to -79
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