Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 8 Issue 1
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- Pages.22-28
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- 1984
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- 1226-5284(pISSN)
The Study on the Freezing Methods and the Cryoprotectants for Rat Embryo Preservation
Rat 수정란의 동결보존에 있어 동결속도 및 동해방지제에 관한 연구
Abstract
This experiment was carried out to investigate effects of DMSO or ethylene glycol as a cryopotectant and of freezing methods on survival rate of forozen-thawed rat 2-cell embryos by morphological observation. 2-cell embryos were recovered from oviducts of Sprague Dawley females mated with males of same strain on day 2 of pregnancy after inducing superovulation by intrapertioneal injection of PMSG and HCG. In slow freezing and thawing groups, embryos were frozen to -79
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