Effects of Dietary Polyunsaturated Fat and Se on Levels of Serum Vitamin E and Creatine Phosphokinase and Morphology of Rat Hepatocytes

Polyunsaturated Fat 및 Se 식이가 흰쥐의 혈청 비타민 E 및 Creatine Phosphokinase 와 간세포의 형태에 미치는 영향

  • Lee, Y.C. (Dept. of Food and Nutrition, Yonsei University) ;
  • Kim, H.Y. (Dept. of Food and Nutrition, Yonsei University) ;
  • Cho, H.Y. (Dept. of Food and Nutrition, Yonsei University) ;
  • Kim, C.S (Dept. of Pathology, Yonsei University) ;
  • Han, S.S (Dept. of Anatomy and Cell Biology, The University of Michigan)
  • 이양자 (연세대학교 가정대학 식생활학과) ;
  • 김혜영 (연세대학교 가정대학 식생활학과) ;
  • 조혜영 (연세대학교 가정대학 식생활학과) ;
  • 김정숙 (연세대학교 의과대학 병리학과) ;
  • 한성수 (미국 미쉬간대학교 해부학 및 세포학교실)
  • Published : 1984.09.30

Abstract

Vitamin E status affected by dietary high PUFA and Se was examined by biochemical and morphological means. Rats were fed four different diets(I : 15% p/s=1 control diet, II : 15% perilla oil diet, III : 15% perilla oil, vitamin mix -vitamin E, IV : 15% perilla oil, vitamin mix-vitamin E and salt mix -Se ) for $4\frac{1}{2}$ weeks. Various dietary treatments had no significant effects on body weight gains of rats. Activities of serum creatine phosphokinase known as an indicator of vitamin E deficiency were significantly higher( P < 0.001) in groups fed diets high in PUFA, regardless of the addition or omission of vitamin E from the vitamin mixture than those in control group. Vitamin E concentrations of serum and liver were affected by experimental diets and serum levels were more affected than those in liver. Electron microscopic observations of the liver revealed 1) the presence of swollen and degenerated mitochondria and lysosome-like body(II), and 2) markedly swollen and degenerated mitochondria, numerous lysosomes and decreased in size and number of microvilli along the bile canaliculus ( III, and 3) a remarkable accumulation of lipid droplets, nuclear pyknosis, degenerated mitochondria and increased number of lysosomes scattered along the cell junction in the hepatocytes (IV).

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