Applied Biological Chemistry
- Volume 27 Issue 2
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- Pages.73-78
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- 1984
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Studies on the Exo-maltotetraohydrolase of Pseudomonas stutzeri IAM 12097 -Part I. Purification of Exo-maltotetraohydrolase-
Pseudomonas stutzeri IAM 12097의 Exo-maltotetraohydrolase에 관한 연구(硏究) -제일보(第一報). Exo-maltotetraohydrolase의 정제(精製)-
- Lee, Mi-Ja (Department of Food Science and Technology, College of Agriculture, Chungbuk National University) ;
- Chung, Man-Jae (Department of Food Science and Technology, College of Agriculture, Chungbuk National University)
- Published : 1984.06.30
Abstract
The optimum culture time and initial pH, for the production of exo-maltotetraohydrolase from Pseudomonas stutzeri IAM 12097, in the trypticase medium were 36 hrs and pH 6.3, respectively. Exo-maltotetraohydrolase was purified by
Pseudomonas stutzeri IAM 12097의 trypticase 배지(培地)에서 36시간(時間), initial pH는 6.3일때 Exo-maltotetraohydrolase가 최대로 생산되었다. Exo-maltotetraohydrolas, 황산(黃酸)암모니아분획(分劃)과 2회(回)의 DEAE-cellulose column chromatography에 의하여 정제(精製)하였으며 정제효소(精製酵素)의 specific activity는 108.6 u/mg protein, 수율(收率)은 9.4%이었다. 본정제효소(本精製酵素)는 polyacrylamide gel electrophoresis와 SDS-polyacrylamide gel electrophoresis에 의하여 각각(各各) 단일(單一) band를 나타내었다.
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