한국균학회지 (The Korean Journal of Mycology)
- 제12권4호
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- Pages.141-152
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- 1984
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
검정곰팡이의 분화에 있어서 고인산뉴클레오티드의 출현 및 8-아자구아닌, 시클로헥시미드의 영향에 관한 연구
Studies on the Occurance of Highly Phosphorylated Nucleotides in the Differentiating Mycelia of Aspergillus niger and Effects of 8-Azaguanine, Cycloheximide on Sporulation
- Kim, Jong-Hyup (Dong-Duck Women's University) ;
- Han, Hee-Jae (Department of Manufacturing Pharmacy, Graduate School, Ehwa Womans University)
- 발행 : 1984.12.30
초록
1. 검정곰팡이 (Aspergillus niger)를 실험재료로 하여 동조적으로 액침배양한 결과 포자의 발아로 부터 균사의 생장, 생식기관의 성숙 및 무성포자의 형성까지를 재현시킬 수 있었다. 2. 각 분화과정에서의 균체로 부터 고인산화뉴를레오티드를 추출하여 P.E.I. Cellulose TLC법으로 전개시켰다. 3. 포자형성 직전의 균체로 부터 얻은 추출물 중에 구아노신테트라포스페이트
Aspergillus niger van Tieghem was cultured by the method of synchronous and submerged culture. Throughout the culture, sporulation was occured. Highly phosphorylated nucleotides in sporulating mycelia were detected to assure whether the eucaryotic Aspergillus niger produce these substances or not during the differentiation. Phosphorylated nucleotides were extracted from the conidiophore, bearing mycelia and spore forming body, these nucleotides were identified by TLC with P.E.I. cellulose. Guanosine tetraphosphate was found in both phialide forming mycelia and spore forming body. The contents of free amino acids were assayed and its level was found to increase at early stage of sporulation. The effects of 8-azaguanine examined, it was found to prevent spore formation and to made abnormal structure. The effects of inosinic monophosphate and guanosine monophosphate on spore formation were examined, spore formation was enhanced by these nucleotides.
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