대한미생물학회지 (The Journal of the Korean Society for Microbiology)
- 제18권1호
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- Pages.53-58
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- 1983
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- 0253-3162(pISSN)
내열성장독소 생산 대장균의 판정
Assay of Heat Stable Enterotoxin Producing E. coli
- 장우현 (서울대학교 의과대학 미생물학교실) ;
- 김문교 (서울대학교 의과대학 미생물학교실) ;
- 최명식 (서울대학교 의과대학 미생물학교실) ;
- 양남웅 (서울대학교 의과대학 미생물학교실) ;
- 고광욱 (서울대학교 의과대학 소아과학교실) ;
- 서정기 (서울대학교 의과대학 소아과학교실)
- Chang, Woo-Hyun (Department of Microbiology, College of Medicine, Seoul National University) ;
- Kim, Moon-Gyo (Department of Microbiology, College of Medicine, Seoul National University) ;
- Choi, Myung-Sik (Department of Microbiology, College of Medicine, Seoul National University) ;
- Yang, Nam-Ung (Department of Microbiology, College of Medicine, Seoul National University) ;
- Ko, Kwang-Wook (Department of Pediatrics, College of Medicine, Seoul National University) ;
- Seo, Jung-Ki (Department of Pediatrics, College of Medicine, Seoul National University)
- 발행 : 1983.12.31
초록
Enterotoxigenic E. coli is one of causative agents of the infantile diarrhea and traveler's diarrhea. A modified infant mouse assay(IMA) was developed for the detection of heat stable enterotoxin (ST) of E. coli isolated from diarrheal and control infants and assay system was established with using enterotoxin producing reference strains. The supernatant of the 24 hour-shaking culture of E. coli in Casamino Acid Yeast Extract Salt Broth(CYES-2) was ingested orally into the 2-4 day old ICR mice. After the mice were kept at
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