Ammonia oxidation activity of nitrifying bacteria and effects of some environmental factors

질화세균의 암모니아 산화능과 이에 미치는 환경요인의 영향

Ammonia oxidation activity of polluted water samples in Jinhae Bay and isolated strain from the seawater was investigated, and effects of environmental factors such as temperature, salinity, substrate concentration to the ammonia oxidation were also investigated. The ammonia oxidation activities of sediments, 0.01-0.04mg eq. $NO_2-N/l/h$, were exceptionally higher than that of sea water, $0.5{\sim}1{\mu}g$ eq. $NO_2-N/l/h$. the activities of muddy sediments at station 4 and 2 were 0.03~0.04mg eq. $NO_2-N/l/h$ and that of sandy sediment at station 3 was 0.002mg eq. $NO_2-N/l/h$. In the case of sea water, the activity of polluted area, station 1, was 2 times higher than that of offshore, station 4. The isolated strain reached log phase after 30days culturs and its oxidation activity was $2{\sim}3{\mu}g$ eq. $NO_2-N/day$. The maximum oxidation of ammonia by IA 13 strain occured at 30mg/l oxidation increased with the salinity rising up to 100% seawater concentraion. And temperature for maximum oxidation of ammonia was $35^{\circ}C$. the oxidation increased with the salinity rising up to 100% seawater concentration.