Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Production of Polysacrhacides by Agrobacterium radiobacter

Agrobacterium radiobacter에 의(依)한 다당류(多糖類) 생산(生産)에 관(關)한 연구(硏究)

  • Lee, Ke-Ho (Department of Food Science and Technology College of Agriculture, Seoul National University) ;
  • Shin, Hyun-Sung (Department of Food Science and Technology College of Agriculture, Seoul National University)
  • 이계호 (서울대학교 농과대학 식품공학과) ;
  • 신현승 (서울대학교 농과대학 식품공학과)
  • Published : 1983.06.30
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Abstract

A slimy non-spore-forming bacterium strain SAF-C isolated from bean stem and root was motile with flagella and identified to one of Agrobacterium radiobacter. Studies were made on the conditions necessary for maximal production of this acidic succinoglucan polysaccharide by this strain in shaken culture. Much production was observed with yeast extract,$(NH_4)_2\;HPO_4$, distillers' dried solubles(D.D.S.), as nitrogen source in the medium composed of 4% glucose, 0.5% nitrogen source, 0.3% $CaCO_3$. The yield was greatest with yeast extract and decreased in order with the above nitrogen source from 22.9% to 9.6 percent. A polysaccharide was produced in a yield of about 25% in a medium composed of 3% glucose, 0.4% D.D.S., 0.5% $K_2\;HPO_4$, 0.01% $MgSO_4{\cdot}7H_2O$.

병(病)든 콩의 줄기와 뿌리에서 분리(分離)한 bacteria균주(菌株) SAF-C는 운동성(運動性)이 있으며 동정(同定)한 결과(結果) Agrobacterium radiobacter임이 밝혀졌다 또 anilineblue 방법(方法)으로 이 균주중(菌株中) 수용성(水溶性) 다당(多糖)만을 거의 완전(完全)하게 생산(生産)하는 균주(菌株)를 선별(選別)하고 SAF-C.W라 하였다. 진탕배양에서 SAF-C.W의 높은 산성(酸性), 수용성다당(水溶性多糖)의 생산조건(生産條件)을 찾기 위하여 본 실험(實驗)이 행(行)하여졌다. 많은 양(量)의 다당(多糖)이 glucose 4%, $CaCO_3$ 0.3% 질소원(窒素源)으로 yeast extract, $(NH_4)_2HPO_4$ 및 distillers dried solubles(D.DS.)을 0.5% 포함(包含)하는 각각의 배지(培地)에서 생산(生産)되었으며, 그 생산율(生産率)은 22.9%, 10%, 9.6%이었다. 또 glucose 3%, D.D.S. 0.4% $K_2HPO_4$ 0.5%, $MgSO_4{\cdot}7H_2O$ 0.01%의 조성(組成)을 가지는 배지(培地)에서 다당수득율(多糖收得率) 25%를 기록(記錄)하였다.

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